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Characterization and Molecular Identification of Unknown Bacteria Isolated From Outlet of Arab El Madabegh Sewage Treatment Plant in Assiut City, Egypt |
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PP: 1-5 |
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Author(s) |
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Momen Salah Kamel Mahmoud,
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Abstract |
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| The aim of this study isolation, characterization and molecular identification of unknown bacteria from the outlet of sewage treatment plant (Arab El Madabegh station) on the Nile River in the Assiut governorate, Egypt. Bacterial strain was isolated and characterized using various biochemical tests and confirmed through molecular approach. Bacterial 16S rRNA gene was amplified using suitable primers. The amplified 16S rRNA gene sequence was compared with the sequence in NCBI sequence database. The bacterial strain was identified as Salmonella .sp m33 (NCBI Gene Bank Accession No: KR027920.1) Phylogenetic and molecular evolutionary analyses were conducted using 16S rRNA sequencing. Salmonella .sp is a gram negative, rode shaped bacteria which forms white raised irregular colony. The sequence when submitted to NCBI gene bank database using BLAST showed 95 – 97% maximum identity and E-value equal to 0 for all closely related taxa. |
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