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Double-Pulse Laser Light Treatment Stimulate Germination and Changes the Oxidative Stress and Antioxidant Activities of Wheat (Triticum aestivum) |
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PP: 1-11 |
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Author(s) |
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Abdelghafar M. Abu-Elsaoud,
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Abstract |
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| Photostimulation using photobiological methods can be considered as a valuable tool for enhancing the agricultural technologies especially in resolving global food problems. Current study aims to investigate the impact of double-pulse He-Ne laser on germination, growth, oxidative stress and antioxidant enzyme of wheat (Triticum aestivum). Wheat grains were irradiated with different doses ranging from 2 to 3600 seconds of He-Ne laser as a double-pulse; once before seed sowing and equal doses after week of seed germination and irradiated to the seedling. The activities of superoxide dismutase (SOD) and hydrogen peroxide (H2O2), and the content of malondialdehyde (MDA) were determined. Results showed remarkably increased germination percentage, germination rate, shoot and root lengths. The results showed that double-pulse He-Ne laser treatment of Egyptian common wheat cultivar could enhance the activities of Cu/Zn-superoxide dismutase (SOD) at low irradiation doses, and lead to a significant decline in response to higher irradiation doses. A significant decline in both hydrogen peroxide and lipid peroxidation (MDA-content) levels were also concluded. It can be concluded that the germination and growth wheat grains were enhanced after seed pretreatment with double shots and oxidative stress were decrease and antioxidant activities enhanced at some irradiation doses. |
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