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Distribution of Actionmycetes in Suez Gulf (Egypt) and Optimization of the Nanoparticles Production of Some Isolates |
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PP: 77- 92 |
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doi:10.18576/jehe/060203
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Author(s) |
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Gehan M. Abou-elela,
Hanan M. Abd-Elnaby,
UsamaAbd-Elraouf,
Moaz. M. Hamed,
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Abstract |
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| Sediment samples were collected from the shallow areas of the Suez Gulf during winter 2014. Eight locations were selected for this study included El-Tour, RasSidr, Suez North, Suez Middle, AdabiaHarbour, Ain Sokhna, RasGharib and RasShokheir. The physico- chemical characters of the samples were determined. The highest count of the acteniomycetes recorded in Adabia harbor (22cfu/gm) and the lowest count recorded in Ain sokhna station (6cfu/gm). Out of 41 actinomycetes isolates, only 11 isolates showed antimicrobial activities against the bacterial pathogens Bacillus subtillus6633, Staphylococcus aureus 25923, Pseudomonas aeruginosa 902 , Bacillus cereus, Salmonella typhimurium 14028, Escherichia coli 19404, and Vibrio damsela. Two isolates identified by molecular techniques as Streptomyces rochei HMM13 and Streptomyces sp., these two isolates were able to produce gold (0.035, 0.013), silver (0.376, 0.355) and zinc (4.080) nanoparticles. Plackett-Burman Design were applied for optimizing the production of nanoparticles by Streptomyces rochei HMM13 against S. typhimurium 14028, E-coli 19404 and Vibrio damsela .The productivity increased by 1.16 , 1.13 and 1.32 fold, respectively. |
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