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Molecular Studies for Myelogenous Leukemia Exposed to Gliding Arc Plasma |
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PP: 41-47 |
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doi:10.18576/ijtfst/140107
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Author(s) |
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A. Elsayed,
A. A. Elhadary,
G. El-Aragi,
F. Fouda,
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Abstract |
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| Gliding Arc Discharge (GAD) non thermal plasma is one type of important new technology in the field of biomedical applications, such as skin disease, wound healing and cancer treatment. This low temperature atmospheric- pressure plasma is a multi-component system that contains such biologically active agents, charged particles, reactive nitrogen and oxygen species, metastable-state molecules or atoms, and UV radiation among other things. The main purpose of this study is to investigate the effect of Gliding Arc Discharge on Acute Myelogenous leukemia cell line (AML). Different doses depend on the time of exposure (40, 60 and 80 sec) applied for one time, the cells going for culture 72 hours. Two types of analysis were carried out. The first one is MTT assay where the viability of cells showed significant decrease in all treated samples, also its realized that increasing the time of exposure leads to good treatment. The second analysis was a molecular proteomics assay for two genes which are caspase-3 and cox-1gene. It was found that exposure to non-thermal plasma gliding arc increases the level of gene transcription (protein product) of caspase-3 gene (Apoptotic gene) which led to more degradation of malignant cells, where the transcription of cox-1 gene (inflammatory gene) reduced.
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