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Biosolubilization Potential of Rock Phosphate and Phosphatase Production by some Phosphate Solubilizing Fungi |
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PP: 277-289 |
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doi:10.18576/jrna/090311
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Author(s) |
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Mohamed G.A,
Susan E. Weesa,
Rofaida S. Abdelkader,
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Abstract |
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| Biomining refers to the utilization of microorganisms to convert metals from rock phosphate ore (RPO) into
soluble compounds, with the aim of extracting elements such as phosphorus. A total of fourteen fungal isolates were
isolated, purified, and tested for their ability to dissolve RPO. The most active isolate was identified as Aspergillus niger
(Assiut University Mycological Center (AUMC) 16252) after extensive testing. A variety of rock phosphate
concentrations, ranging from 5 g/L to 400 g/L, were investigated. In response to gamma radiation exposure, many
isolates, but especially A. niger, increased phosphatase activity. A. niger showed the highest activity, which increased
even at high radiation gamma doses. A. niger possess the highest value of solubilized P under static condition (34.3 %
P2O5). Flow culture studies have revealed that the capacity of A. niger to create phosphatases is regulated by growth rate,
phosphate availability, and pH. When A. niger was cultured under static conditions at 28 °C, the specific activities of
Acidic phosphatase and Alkaline phosphatase attained maximum values of 21 and 23.2 mg/mL, respectively. The pH of
the substrate raised to 7 following the addition of rock phosphate and subsequently reduced to 5.02 after A. niger were
infected and cultured for 3 days. These results imply that some fungus with less reactive RP can be utilized as natural
fertilizers. This can help reduce the demand for synthetic fertilizers while also improving the environment and soil
quality. |
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