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Studying The Effect of The Dielectric Barrier Discharge Non- thermal Plasma on Colon Cancer Cell line |
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PP: 161-168 |
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doi:10.18576/ijtfst/100305
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Author(s) |
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Abdelmonsef A. Elhadary,
A. El-Zein,
M. Talaat,
G. El-Aragi,
A. El-Amawy,
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Abstract |
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| Non-thermal plasma is a future tool for the treatment of cancer, some diseases, and for many medical applications. Dielectric Barrier Discharge Plasma (DBDP) is a type of plasma that has been used for the treatment of cancers in different researches. In this work (DBDP) was used to evaluate its effect on Colon Cancer Cell-line (CaCo Cell- line), through three doses depending on the time of exposure (40, 60, and 80 sec.). After exposure, the cell-line were cultured for 72 hours and followed by two types of analysis. The first was MTT assay for the determination of cell viability. The second was molecular analysis for two genes associated with cancers; Caspase 9 gene (apoptotic gene) and Bax gene (pro-apoptotic gene) through detection of their proteins. The results of the study proved that the DBD Plasma has a strong killing effect on the cancer cell-line (CaCo), and this effect increased with the time of exposure increased. The study proved also that; the effect of DBDP coming from its effect on the transcription of genes associated with cancer, where the study found that the degree of transcription of Caspase 9 and Bax genes was significantly increased as the result of the exposure. Therefore, the results of this study make us consider that DBD Plasma Device is a promised tool for the treatment of cancer, where the plasma promotes the process of apoptosis in cancer cells.
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