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Impact of Diazotrophic Bacteria on Germination and Growth of Tomato, with Bio-control Effect, Isolated from Algerian Soil |
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PP: 1-7 |
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Author(s) |
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N. Tabli,
E H. Nabti,
D. Dahel,
N. Mokrane,
H. Manyani,
M. G. Megias,
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Abstract |
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| 10 soils samples were collected from the west region of Bejaia-Algeria in aseptic conditions. The preliminary germination
test on tomatos seeds allowed screening of 4 simples from which 40 different colonies were isolated and purified. These last were
submitted to in vitro germination test, while 20 strains with an inductor effect on the germination were selected and tested for the
presence of stimulator effect of growth of tomato in vivo. Biochemical (on API 50 CHB/E gallery), and phylogenetic (DNAr 16S
sequencing) identification, tests researching characteristics promoting plant growth (production of IAA (Indol Acetic Acid) phytohormone,
enzymes, siderophores, free nitrogen fixation, and an antagonistic test on five phytopathogenic fungi (Botrytis cinerea;
Phytophtora cactorum, phytophtora cinnamomi; Fusarium oxysporum and Verticillium dahliae) were applied for the strain showing
positive in vivo germination results on tomato.The results of this last test led to select four strains: S11, S6, S15, and S12 that greatly
affected tomato growth, with stems length (cm) of 9.212, 9.280, 9.362 and 9.858, respectively, compared to the control (6.400).
Biochemical and phylogenic identifications of these strains allowed to affiliate themas following: Arthrobacter agilis (S6), Streptomyces
Sp (S11), Bacillus sp13 (S15) and Mycobacterium Sp (S12). The four strains produced several enzymes, high indol acetic acid (phytohormone)
quantity (mg/l: 38.47 (S11); 86.90 (S6); 93.96 (S12) and 190.23 (S15), siderophores, nitrogen fixing. It also showed an
antagonistic activity against the phytopathogenic fungi tested with variable plant growth inhibition (PGI) %:0-78.51% for Bacillus
sp13; 29-74%for Streptomyces sp; 0-72%for Arthrobacter agilis; and 0-69% for Mycobacterium sp. These results prove that the strains
are compatible for tomato growth stimulation. |
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